Protopine reduces the inflammatory activity of lipopolysaccharide-stimulated murine macrophages

Posted by on Dec 20, 2012 in Uncategorized | No Comments

BMB Rep. 2012 Feb;45(2):108-13. doi: 10.5483/BMBRep.2012.45.2.108.

Bae DS, Kim YH, Pan CH, Nho CW, Samdan J, Yansan J, Lee JK.

Department of Biology Education, College of Education, Chungbuk National University, Cheongju 361-763, Korea.


Protopine is an isoquinoline alkaloid contained in plants in northeast Asia. In this study, we investigated whether protopine derived from Hypecoum erectum L could suppress lipopolysaccharide (LPS)-induced inflammatory responses in murine macrophages (Raw 264.7 cells). Protopine was found to reduce nitric oxide (NO), cyclooxygenase-2 (COX-2), and prostaglandin E(2) (PGE(2)) production by LPS-stimulated Raw 264.7 cells, without a cytotoxic effect. Pre-treatment of Raw 264.7 cells with protopine reduced the production of pro-inflammatory cytokines. These inhibitory effects were caused by blocking phosphorylation of mitogen-activated protein kinases (MAP kinases) and also blocking activation of a nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB).